Reaction Process: Plant Reactome:R-TAE-1119456
Brassinosteroid biosynthesis II related metabolites
find 7 related metabolites which is associated with chemical reaction(pathway) Brassinosteroid biosynthesis II
(22S)-22-hydroxycampest-4-en-3-one + H+ + TPNH ⟶ (22alpha)-hydroxy-5alpha-campestan-3-one + TPN
Nicotinamide adenine dinucleotide phosphate
NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. Nicotinamide adenine dinucleotide phosphate (NADP) is a coenzyme composed of ribosylnicotinamide 5-phosphate (NMN) coupled with a pyrophosphate linkage to 5-phosphate adenosine 2,5-bisphosphate. NADP serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). NADP is formed through the addition of a phosphate group to the 2 position of the adenosyl nucleotide through an ester linkage (Dorland, 27th ed). This extra phosphate is added by the enzyme NAD+ kinase and removed via NADP+ phosphatase. NADP is also known as TPN (triphosphopyridine nucleotide) and it is an important cofactor used in anabolic reactions in all forms of cellular life. Examples include the Calvin cycle, cholesterol synthesis, fatty acid elongation, and nucleic acid synthesis (Wikipedia). Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5-phosphate (NMN) coupled by pyrophosphate linkage to the 5-phosphate adenosine 2,5-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed.) [HMDB]. NADPH is found in many foods, some of which are american pokeweed, rice, ginseng, and ostrich fern. COVID info from COVID-19 Disease Map Corona-virus Coronavirus SARS-CoV-2 COVID-19 SARS-CoV COVID19 SARS2 SARS
NADP+
[C21H29N7O17P3]+ (744.0832754)
[Spectral] NADP+ (exact mass = 743.07545) and NAD+ (exact mass = 663.10912) were not completely separated on HPLC under the present analytical conditions as described in AC$XXX. Additionally some of the peaks in this data contains dimers and other unidentified ions. COVID info from COVID-19 Disease Map Corona-virus Coronavirus SARS-CoV-2 COVID-19 SARS-CoV COVID19 SARS2 SARS
5alpha-Campestan-3-one
5alpha-Campestan-3-one, also known as 3-dehydro-campestanol or methylcholestanone, belongs to the class of organic compounds known as ergosterols and derivatives. These are steroids containing ergosta-5,7,22-trien-3beta-ol or a derivative thereof, which is based on the 3beta-hydroxylated ergostane skeleton. Thus, 5alpha-campestan-3-one is considered to be a sterol lipid molecule. 5alpha-Campestan-3-one is a very hydrophobic molecule, practically insoluble (in water), and relatively neutral. 5alpha-Campestan-3-one is involved in the brassinosteroid biosynthesis pathway. 5alpha-Campestan-3-one is produced from campest-4-en-3-one by the action of DET2 (EC 1.3.99.-), a probable steroid reductase. 5alpha-Campestan-3-one is then converted into campestanol or 22alpha-hydroxy-5alpha-campestan-3-one. The conversion to 22alpha-hydroxy-5alpha-campestan-3-one is catalyzed by DWF4, a steroid 22-alpha-hydroxylase (EC 1.14.13.-).
Campest-4-en-3-one
Campest-4-en-3-one is an intermediate in Brassinolide Biosynthesis pathway.The conversion of the membrane Sterol Campesterol to BL occurs via a series of reductions, hydroxylations, epimerizations and oxidations that have been extensively studied in several species. The conversion of Campesterol to Campestanol is not a single step, but composed of the biosynthetic sequence of Campesterol 4-en-3Beta-ol ---> 4-en-3-one ---> 3-one ---> Campestanol in Arabidopsis. In the first step, Campesterol is converted to Campest-4-en-3Beta-ol in presence of enzyme Delta-5-3-Ketosteroid Isomerase. Enzymes that catalyze the conversion from 3-Beta-Hydroxy-Delta,5-6-Steroid to 3-oxo-Delta-4-5 Isomerase have been reported in Bacteria and Mammals. Campest-4-en-3 Beta-ol is converted to Campest-4-en-3-one in presence of enzyme 3-Beta-Hydroxysteroid Dehydrogenase. In the next step, Campest-4-en-3-one is converted to Campestanol via 5-Alpha-Campestan-3-one. 3-Oxo-5Alpha-Steroid 4-Dehydrogenase family members (encoded by Det2 in Arabidopsis) catalyze the formation of 5-Alpha-Campestan-3-one. Isoo. from Phoenix dactylifera (date). (24R)-Ergost-4-en-3-one is found in many foods, some of which are rowanberry, chicory leaves, common hazelnut, and common thyme.
(22alpha)-Hydroxy-campest-4-en-3-one
(22alpha)-Hydroxy-campest-4-en-3-one belongs to the class of organic compounds known as monohydroxy bile acids, alcohols, and derivatives. These are bile acids, alcohols, or any of their derivatives bearing a hydroxyl group. Thus, (22alpha)-hydroxy-campest-4-en-3-one is considered to be a sterol lipid molecule. (22alpha)-Hydroxy-campest-4-en-3-one is a very hydrophobic molecule, practically insoluble (in water), and relatively neutral. (22alpha)-Hydroxy-campest-4-en-3-one is involved in the brassinosteroid biosynthesis pathway. (22alpha)-Hydroxy-campest-4-en-3-one is created from either campest-4-en-3-one or 22alpha-hydroxy-campesterol through the actions of steroid 22-alpha-hydroxylase (EC 1.14.13.-) or Sax1, respectively. (22alpha)-Hydroxy-campest-4-en-3-one is then converted into 22alpha-hydroxy-5alpha-campestan-3-one by steroid reductase DET2 (EC 1.3.99.-).
22alpha-Hydroxy-5alpha-campestan-3-one
22alpha-Hydroxy-5alpha-campestan-3-one belongs to the class of organic compounds known as monohydroxy bile acids, alcohols, and derivatives. These are bile acids, alcohols, or any of their derivatives bearing a hydroxyl group. Thus, 22alpha-hydroxy-5alpha-campestan-3-one is considered to be a sterol lipid molecule. 22alpha-Hydroxy-5alpha-campestan-3-one is involved in the brassinosteroid biosynthesis pathway (brassinosteroids are found in plants). 22alpha-Hydroxy-5alpha-campestan-3-one is produced from either 5alpha-campestan-3-one or 22alpha-hydroxy-campest-4-en-3-one, through the function of steroid 22-alpha-hydroxylase (EC 1.14.13.-) or steroid reductase DET2 (EC 1.3.99.-), respectively. 22alpha-Hydroxy-5alpha-campestan-3-one is then converted into 6-deoxocathasterone.
Hydrogen Ion
Hydrogen ion, also known as proton or h+, is a member of the class of compounds known as other non-metal hydrides. Other non-metal hydrides are inorganic compounds in which the heaviest atom bonded to a hydrogen atom is belongs to the class of other non-metals. Hydrogen ion can be found in a number of food items such as lowbush blueberry, groundcherry, parsley, and tarragon, which makes hydrogen ion a potential biomarker for the consumption of these food products. Hydrogen ion exists in all living organisms, ranging from bacteria to humans. In humans, hydrogen ion is involved in several metabolic pathways, some of which include cardiolipin biosynthesis cl(i-13:0/a-25:0/a-21:0/i-15:0), cardiolipin biosynthesis cl(a-13:0/a-17:0/i-13:0/a-25:0), cardiolipin biosynthesis cl(i-12:0/i-13:0/a-17:0/a-15:0), and cardiolipin biosynthesis CL(16:1(9Z)/22:5(4Z,7Z,10Z,13Z,16Z)/18:1(11Z)/22:5(7Z,10Z,13Z,16Z,19Z)). Hydrogen ion is also involved in several metabolic disorders, some of which include de novo triacylglycerol biosynthesis TG(20:3(8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:5(7Z,10Z,13Z,16Z,19Z)), de novo triacylglycerol biosynthesis TG(18:2(9Z,12Z)/20:0/20:4(5Z,8Z,11Z,14Z)), de novo triacylglycerol biosynthesis TG(18:4(6Z,9Z,12Z,15Z)/18:3(9Z,12Z,15Z)/18:4(6Z,9Z,12Z,15Z)), and de novo triacylglycerol biosynthesis TG(24:0/20:5(5Z,8Z,11Z,14Z,17Z)/24:0). A hydrogen ion is created when a hydrogen atom loses or gains an electron. A positively charged hydrogen ion (or proton) can readily combine with other particles and therefore is only seen isolated when it is in a gaseous state or a nearly particle-free space. Due to its extremely high charge density of approximately 2×1010 times that of a sodium ion, the bare hydrogen ion cannot exist freely in solution as it readily hydrates, i.e., bonds quickly. The hydrogen ion is recommended by IUPAC as a general term for all ions of hydrogen and its isotopes. Depending on the charge of the ion, two different classes can be distinguished: positively charged ions and negatively charged ions . Hydrogen ion is recommended by IUPAC as a general term for all ions of hydrogen and its isotopes. Depending on the charge of the ion, two different classes can be distinguished: positively charged ions and negatively charged ions. Under aqueous conditions found in biochemistry, hydrogen ions exist as the hydrated form hydronium, H3O+, but these are often still referred to as hydrogen ions or even protons by biochemists. [Wikipedia])